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A High-throughput Cre-Lox Activated Viral Membrane Fusion

In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter. In Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Site Terms and Conditions | Cookie Options If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at “High” 2 ml of 10% Bleach for 10 minutes provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer.

Default Filters for Configuration BD_LSR-Fortessa_18-COLOR_20181211 Laser Detector Name Dichroic Mirror Bandpass Filter Sample Parameters / Fluorochromes 355C-379 None 379/28 BUV395 355B-474 450LP 474/25 CellTrace calcein Blue AM; L/D/F Blue; DC Violet; Hoechst 33342; Hoechst 33258; DAPI (best) 355A-730 685 LP 730/45 BUV737; Qdot 705 405E-450 Optical filters are available that are suitable for BD FACSCelesta™ and BD LSRFortessa™ X-20 polygon detector arrays as well as for BD LSRFortessa™ and BD FACSAria™ product family octagon and trigon detector arrays. BD™ Optical Filters Optimal detection of BD Horizon Brilliant™ Polymer Conjugates Band Filter Holder. Status: Cat No.:336102. Quick View. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).

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Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) Default Filters for Configuration BD_LSR-Fortessa_18-COLOR_20181211 Laser Detector Name Dichroic Mirror Bandpass Filter Sample Parameters / Fluorochromes Default Filters for Configuration BD_LSR-Fortessa_18-COLOR_20181211 Laser Detector Name Dichroic Mirror Bandpass Filter Sample Parameters / Fluorochromes 355C-379 None 379/28 BUV395 355B-474 450LP 474/25 CellTrace calcein Blue AM; L/D/F Blue; DC Violet; Hoechst 33342; Hoechst 33258; DAPI (best) 355A-730 685 LP 730/45 BUV737; Qdot 705 405E-450 BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor arrays. The BD LSRFortessa uses BD’s patented octagon- and trigon-shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options.

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Performance Fluorescence Sensitivity Measurements performed using SPHERO™ Rainbow Calibration Particles RCP-30-5A. Fortessa A. 355nM UV. Collection filter Mirror Fluorochromes; 450/50- Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Core Facility, BBB BD LSR Fortessa, filter configurations and fluorochromes Marianne Enger Side 1 12.06.2013 LASER PMT LP FILTER BP FILTER FLUOROCHROMES (examples) A 685 695/40 PerCP-Cy5.5 488 (blue) D B 635 670/30 PerCP C 505 530/30 FITC, eGFP, eYFP, DiO, CFSE, YoPro1, Alexa488 - 488/10 SSC FSC 1 inch filter cassette v10.x3d Rating Select rating Give BD Aria/LSRII/Fortessa optical filter holders 1/5 Give BD Aria/LSRII/Fortessa optical filter holders 2/5 Give BD Aria/LSRII/Fortessa optical filter holders 3/5 Give BD Aria/LSRII/Fortessa optical filter holders 4/5 BD FACSCelesta ™ multicolor cell analyzer .

Fluorochromes (Some). 355nM.
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Sample Injection Port (SIP):(Tube support arm) SIP has two positions. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig.

Designed for limited space and boundless potential.
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BUV 395. UV C. Purge bubbles from sheath filter if necessary. BD Tech Bulletin: Multicolor compensation · BD Application Note: Selecting reagents for multicolor experiments The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's 2012 BD. Relative. Brightness.